Article
Primary reaction of sensory rhodopsin II mutant D75N and the influence of azide.
Biochemistry - 13 Oct 2009
Verhoefen Mirka-Kristin, Lenz Martin O, Amarie Sergiu, Klare Johann P, Tittor Jörg, Oesterhelt Dieter, Engelhard Martin, Wachtveitl Josef
Abstract excerpt
The early steps in the photocycle of sensory rhodopsin II mutant D75N are investigated in a comprehensive study using femtosecond visible pump/probe spectroscopy. An overall slower response dynamics after photoexcitation is observed compared to wild-type sensory rhodopsin II, which is assigned to changed electrostatics and an altered hydrogen-bonding network within the retinal binding pocket. Furthermore, the...
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