A mutant rhodopsin photoproduct with a protonated Schiff base displays an active-state conformation: a Fourier-transform infrared spectroscopy study.

doi:10.1021/bi00250a021

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Article

A mutant rhodopsin photoproduct with a protonated Schiff base displays an active-state conformation: a Fourier-transform infrared spectroscopy study.

Biochemistry - 22 Nov 1994

Fahmy K, Siebert F, Sakmar T P

Abstract excerpt

In the rhodopsin mutant E113A/A117E the position of the protonated Schiff base counterion, Glu113, is moved by one helix turn from position 113 to 117. The photoreaction of this mutant pigment was studied by Fourier-transform infrared (FTIR) difference spectroscopy. At acidic pH, formation of a 4...

Topics

Carboxylic Acids
Hydrogen Bonding
Mutation
Photochemistry
Protein Conformation
Protons
Rhodopsin
Schiff Bases
Spectroscopy, Fourier Transform Infrared

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