Article
Primary reaction dynamics of proteorhodopsin mutant D97N observed by femtosecond infrared and visible spectroscopy.
Photochemistry and photobiology - 1 Jan 2000
Verhoefen Mirka-Kristin, Neumann Karsten, Weber Ingrid, Glaubitz Clemens, Wachtveitl Josef
Abstract excerpt
Femtosecond time-resolved spectroscopy in the visible and IR range was utilized to study the primary reaction dynamics of the proteorhodopsin (PR) D97N mutant in comparison with wild type PR at different pH values. The analysis of the data obtained in the mid-IR closely resembles the results for wild type PR. The observation of the first ground state intermediate K is initially obscured by a complex reaction...
Topics
- Aspartic Acid
- Mutation
- Rhodopsin
- Rhodopsins, Microbial
- Spectrum Analysis
- Time Factors
