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Article

Structural impact of the E113Q counterion mutation on the activation and deactivation pathways of the G protein-coupled receptor rhodopsin.

Journal of molecular biology - 27 Jun 2008

Standfuss Jörg, Zaitseva Ekaterina, Mahalingam Mohana, Vogel Reiner

Abstract excerpt

Disruption of an interhelical salt bridge between the retinal protonated Schiff base linked to H7 and Glu113 on H3 is one of the decisive steps during activation of rhodopsin. Using previously established stabilization strategies, we engineered a stabilized E113Q counterion mutant that converted rhodopsin to a UV-absorbing photoreceptor with deprotonated Schiff base and allowed reconstitution into native-like...

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Identifiers

PMID: 18511075
DOI: 10.1016/j.jmb.2008.04.055
PMCID: PMC2726285

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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