Article
Virulence gene deletion frequency is increased in Shigella flexneri following conjugation, transduction, and transformation.
FEMS microbiology letters - 1 Feb 1997
Porter M E, Dorman C J
Abstract excerpt
Some commonly used methods for introducing DNA provoke spontaneous loss of expression of a virulence gene located on the high molecular mass plasmid in Shigella flexneri. The introduction of plasmid DNA by calcium chloride-mediated transformation in strains harbouring wild-type or mutated copies...
Topics
- Analysis of Variance
- Blotting, Northern
- Blotting, Southern
- Calcium Chloride
- Conjugation, Genetic
- DNA, Bacterial
- Electroporation
- Gene Deletion
- Gene Expression Regulation, Bacterial
- Genetic Complementation Test
- Lac Operon
- Mutation
- Phenotype
- Plasmids
- Shigella flexneri
- Transformation, Genetic
- Virulence