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Article

Virulence gene deletion frequency is increased in Shigella flexneri following conjugation, transduction, and transformation.

FEMS microbiology letters - 1 Feb 1997

Porter M E, Dorman C J

Abstract excerpt

Some commonly used methods for introducing DNA provoke spontaneous loss of expression of a virulence gene located on the high molecular mass plasmid in Shigella flexneri. The introduction of plasmid DNA by calcium chloride-mediated transformation in strains harbouring wild-type or mutated copies of regulatory genes resulted in the loss of expression of an mxiC-lacZ reporter gene at high frequency, approaching...

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Identifiers

PMID: 9037774
DOI: 10.1111/j.1574-6968.1997.tb10237.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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