Article
Identification and characterization of virK, a virulence-associated large plasmid gene essential for intercellular spreading of Shigella flexneri.
Molecular microbiology - 1 Aug 1992
Nakata N, Sasakawa C, Okada N, Tobe T, Fukuda I, Suzuki T, Komatsu K, Yoshikawa M
Abstract excerpt
Seven virulence loci have been identified by Tn5 insertion mutagenesis on the large 230 kb plasmid (pMYSH6000) of Shigella flexneri 2a. In this study, we used Tn10 insertion mutagenesis and identified a novel virulence locus on pMYSH6000 responsible for bacterial spread. Characterization of the i...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cell Movement
- DNA, Bacterial
- Deoxyribonucleases, Type II Site-Specific
- Immunoblotting
- Molecular Sequence Data
- Mutagenesis, Insertional
- Phenotype
- Plasmids
- Restriction Mapping
- Shigella flexneri
- Virulence