Article
Creation of stable Pseudomonas aeruginosa promoter-reporter fusion mutants using linear plasmid DNA transformation.
BMC research notes - 24 Jun 2016
Chen Ping, Leung Kai P
Abstract excerpt
BACKGROUND: Pseudomonas aeruginosa is an important opportunistic human pathogen that is commonly encountered clinically in different types of infections. Reporter-gene systems and construction of mutants defective in specific functions are useful tools for studying the cellular physiology and virulence of this organism. The common mutant construction process requires constructing target alleles into large size...
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