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Article

Development of efficient suicide mechanisms for biological containment of bacteria.

Applied and environmental microbiology - 1 Jan 1991

Knudsen S M, Karlström O H

Abstract excerpt

To optimize plasmid containment, we have systematically investigated the factors that limit the killing efficiency of a suicide system based on the relF gene from Escherichia coli controlled by inducible lac promoters and placed on plasmids. In induction experiments with this suicide system, killing efficiency was unaffected by temperature and growth medium; there was no requirement for great promoter strength or...

Topics

Bacteria
Conjugation, Genetic
Containment of Biohazards
Genes, Bacterial
Genes, Lethal
Genetic Engineering
Mutation
Plasmids
Transduction, Genetic
Transformation, Genetic

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Identifiers

PMID: 2036024
DOI: 10.1128/aem.57.1.85-92.1991
PMCID: PMC182668

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2036024-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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