Article
Multivariant confirmation of sickle cell disease using a non-radioactive minisequencing reaction.
Hemoglobin - 1 Jan 1997
Tuuminen T, Ingman H, Therrell B L, Kallio A
Abstract excerpt
A non-radioactive solid-phase minisequencing method for confirmation of abnormal hemoglobin variants causing sickle cell disease has been developed. In this method amplified 5'-biotinylated target sequences containing normal and mutation sites are immobilized onto streptavidin-coated microplates....
Topics
- Anemia, Sickle Cell
- Diagnosis, Differential
- Enzyme-Linked Immunosorbent Assay
- Fluorometry
- Hemoglobins, Abnormal
- Humans
- Mutation
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Sequence Analysis, DNA
- Single-Blind Method