Article
Minisequencing on functionalised self-assembled monolayer as a simple approach for single nucleotide polymorphism analysis of cattle.
Zeitschrift fur Naturforschung. C, Journal of biosciences - 1 Jan 2000
Du Weidong, Cieplik Michael, Durstewitz Gregor, Sarkar Chandra Mohan, Kruschina Margit, Fries Rudi, Ortigao Flavio Ramalho
Abstract excerpt
We have developed a genetic barcode module, based on a parallel sorting facility of single nucleotide polymorphism for secure individual identification of cattle. Biotinylated allele-specific oligonucleotides were immobilized onto the predefined spots of streptavidin tethered self-assembled monolayers with long chain alkanethiols on biochips. The target DNAs for hybridization and subsequent on-chip minisequencing...
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