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Efficient modification of human chromosomal alleles using recombination-proficient chicken/human microcell hybrids.

Nature genetics - 1 Feb 1996

Dieken E S, Epner E M, Fiering S, Fournier R E, Groudine M

Abstract excerpt

Targeted modification of human chromosomal alleles by homologous recombination is a powerful approach to study gene function, but gene targeting in mammalian cells is an inefficient process. In contrast, gene targeting in a chicken pre-B cell line, DT40, is highly efficient. We have transferred human chromosome 11 into DT40 cells by microcell fusion, and find that the resulting hybrids are...

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PMID: 8563756
DOI: 10.1038/ng0296-174

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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