Article
Direct selection of hepatoma cell variants deficient in alpha 1-antitrypsin gene expression.
Somatic cell and molecular genetics - 1 Jul 1992
Bulla G A, Fournier R E
Abstract excerpt
Expression plasmids containing the human alpha 1-antitrypsin (alpha 1 AT) promoter fused to either adenine phosphoribosyltransferase (aprt) or xanthine-guanine phosphoribosyltransferase (gpt) coding sequences were sequentially introduced into APRT- HPRT- rat hepatoma cells. Stable transfectants e...
Topics
- Adenine Phosphoribosyltransferase
- Animals
- Carcinoma, Hepatocellular
- DNA, Neoplasm
- Genetic Variation
- Humans
- Liver Neoplasms
- Pentosyltransferases
- Phenotype
- Promoter Regions, Genetic
- RNA, Messenger
- Rats
- Selection, Genetic
- Transcription, Genetic
- Transfection
- Tumor Cells, Cultured
- alpha 1-Antitrypsin