Article
Development of improved tRNAs for in vitro biosynthesis of proteins containing unnatural amino acids.
Chemistry & biology - 1 Dec 1996
Cload S T, Liu D R, Froland W A, Schultz P G
Abstract excerpt
BACKGROUND: Chemically aminoacylated suppressor tRNAs have previously been used in vitro to generate mutant proteins in which unnatural amino acids are incorporated site-specifically. Although the existing methodology often provides adequate quantities of mutant proteins, the suppression efficiencies of some unnatural amino acids are not high enough to yield useful amounts of protein. In an effort to make this...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.