Article
The last two cytoplasmic loops in the lactose permease of Escherichia coli comprise a discontinuous epitope for a monoclonal antibody.
Biochemistry - 7 Jan 1997
Sun J, Li J, Carrasco N, Kaback H R
Abstract excerpt
Monoclonal antibody (mAb) 4B11 binds to a conformational epitope in the lactose permease that is exposed on the cytoplasmic face of the membrane with a KD of 2.8 x 10(-7) M. By studying binding of 4B11 to permease mutants containing six contiguous His residues in each cytoplasmic loop, inserted f...
Topics
- Antibodies, Monoclonal
- Avidin
- Biotin
- Blotting, Western
- Carrier Proteins
- Cell Membrane
- Epitopes
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Kinetics
- Membrane Proteins
- Membrane Transport Proteins
- Monosaccharide Transport Proteins
- Mutagenesis, Site-Directed
- Mutation
- Protein Binding
- Protein Conformation