Article
Study by mutagenesis of the roles of two aromatic clusters of alpha-lactalbumin in aspects of its action in the lactose synthase system.
The Journal of biological chemistry - 18 Feb 1994
Grobler J A, Wang M, Pike A C, Brew K
Abstract excerpt
A new system for the bacterial expression of a variant of bovine alpha-lactalbumin has been developed. Eighteen mutant proteins containing single site substitutions in a cluster of predominantly aromatic residues adjacent to the cleft (aromatic cluster I) and in the hydrophobic box were expressed...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Cattle
- Chromatography, Gel
- Chromatography, High Pressure Liquid
- Chromatography, Ion Exchange
- Circular Dichroism
- DNA Primers
- Galactosyltransferases
- Genetic Variation
- Glucose
- Kinetics
- Lactalbumin
- Lactose Synthase
- Mathematics
- Models, Molecular