Helices VII and X in the lactose permease of Escherichia coli: proximity and ligand-induced distance changes.

Abstract excerpt

By using functional lactose permease devoid of native Cys residues with a discontinuity in the periplasmic loop between helices VII and VIII (N(7)/C(5) split permease), cross-linking between engineered paired Cys residues in helices VII and X was studied with the homobifunctional, thiol-specific cross-linkers 1,1-methanediyl bismethanethiosulfonate (3 A), N,N'-o- phenylenedimaleimide (6 A) and...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Biological Transport, Active
• Share to Cross-Linking Reagents
• Share to Cysteine