Article
Identification of biologically active mutants by combinatorial cassette mutagenesis: exclusion of wild-type codon from degenerate codons.
Analytical biochemistry - 1 May 1994
Huang W, Santi D V
Abstract excerpt
A degenerate codon (N)(N)(G+C) is often used in cassette mutagenesis to encode all 20 natural amino acids at the target mutation site. However, the presence of the wild-type codon in the degenerate codon presents some inconvenience in screening and identification of catalytically active mutants. The wild-type enzyme will always be identified as catalytically active in a screening process and in most cases can...
Topics
- Amino Acids
- Base Sequence
- Codon
- DNA
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation
- Oligonucleotides
- Sequence Analysis, DNA