Isolation and in vitro characterization of temperature-sensitive mutants of the bacteriophage f1 gene V protein.

doi:10.1016/0022-2836(91)90566-o

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Article

Isolation and in vitro characterization of temperature-sensitive mutants of the bacteriophage f1 gene V protein.

Journal of molecular biology - 20 May 1991

Zabin H B, Terwilliger T C

Abstract excerpt

In vivo selections were used to isolate 43 temperature-sensitive gene V mutants of the bacteriophage f1 from a collection of mutants constructed by saturation mutagenesis of the gene. The sites of temperature-sensitive substitutions are found in both the beta-sheets and the turns of the protein,...

Topics

Codon
Coliphages
Escherichia coli
Genes, Viral
Genetic Variation
Kinetics
Mutagenesis
Oligonucleotide Probes
Phenotype
Protein Conformation
Temperature
Viral Proteins

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