Article
Probing druggability and biological function of essential proteins in Leishmania combining facilitated null mutant and plasmid shuffle analyses.
Molecular microbiology - 1 Jul 2014
Dacher Mariko, Morales Miguel A, Pescher Pascale, Leclercq Olivier, Rachidi Najma, Prina Eric, Cayla Mathieu, Descoteaux Albert, Späth Gerald F
Abstract excerpt
Leishmania parasites cause important human morbidity and mortality. Essential Leishmania genes escape genetic assessment by loss-of-function analyses due to lethal null mutant phenotypes, even though these genes and their products are biologically most significant and represent validated drug targets. Here we overcome this limitation using a facilitated null mutant approach applied for the functional genetic...
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