Article
Cloning of the promoters of an Escherichia coli rRNA gene. New experimental system to study the regulation of rRNA transcription.
Gene - 1 Jan 2000
Boros I, Kiss A, Sain B, Somlyai G, Venetianer P
Abstract excerpt
The promoters of the rrnB gene of Escherichia coli have been cloned on a multicopy, pBR322-derived plasmid by deleting most of the structural part of rrnB and fusing the terminators of the gene immediately to the promoters. Several further deletions were constructed to vary the promoter-terminator distance, destroy or damage selectively any of the promoters or terminators, and vary the distance between the two...
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