Cloning of the promoters of an Escherichia coli rRNA gene. New experimental system to study the regulation of rRNA transcription.

doi:10.1016/0378-1119(83)90103-8

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Article

Cloning of the promoters of an Escherichia coli rRNA gene. New experimental system to study the regulation of rRNA transcription.

Gene - 1 Jan 2000

Boros I, Kiss A, Sain B, Somlyai G, Venetianer P

Abstract excerpt

The promoters of the rrnB gene of Escherichia coli have been cloned on a multicopy, pBR322-derived plasmid by deleting most of the structural part of rrnB and fusing the terminators of the gene immediately to the promoters. Several further deletions were constructed to vary the promoter-terminato...

Topics

Chromosome Deletion
Chromosomes, Bacterial
Cloning, Molecular
DNA Restriction Enzymes
Escherichia coli
Genes
Mutation
Operon
Plasmids
RNA, Ribosomal
Transcription, Genetic

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