Article
A broad-host-range cloning vector transposable to various replicons.
Gene - 1 Jan 2000
Grinter N J
Abstract excerpt
A system is described for the stable insertion of cloned DNA sequences into the chromosomes of Gram-negative bacteria. Two broad-host-range plasmids form the basis of the system: one (the "carrier") contains a transposable DNA sequence into which foreign DNA can be cloned; the second (the "helper") provides transposition functions in trans. Both plasmids can be readily transferred between Gram-negative bacteria...
Topics
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Transposable Elements
- Escherichia coli
- Euryarchaeota
- Genes, Bacterial
- Phenotype
- Plasmids
- Pseudomonas aeruginosa
- Replicon
- Species Specificity