Article
A broad-host-range cloning vector transposable to various replicons.
Gene - 1 Jan 2000
Grinter N J
Abstract excerpt
A system is described for the stable insertion of cloned DNA sequences into the chromosomes of Gram-negative bacteria. Two broad-host-range plasmids form the basis of the system: one (the "carrier") contains a transposable DNA sequence into which foreign DNA can be cloned; the second (the "helper") provides transposition functions in trans. Both plasmids can be readily transferred between Gram-negative bacteria...
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