Article
A chromosomal insertion toolbox for promoter probing, mutant complementation, and pathogenicity studies in Ralstonia solanacearum.
Molecular plant-microbe interactions : MPMI - 1 Apr 2012
Monteiro Freddy, Solé Montserrat, van Dijk Irene, Valls Marc
Abstract excerpt
We describe here the construction of a delivery system for stable and directed insertion of gene constructs in a permissive chromosomal site of the bacterial wilt pathogen Ralstonia solanacearum. The system consists of a collection of suicide vectors-the Ralstonia chromosome (pRC) series-that carry an integration element flanked by transcription terminators and two sequences of homology to the chromosome of...
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