Single-step conjugative cloning of bacterial gene fusions involved in microbe-host interactions.

doi:10.1007/s004380050548

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Article

Single-step conjugative cloning of bacterial gene fusions involved in microbe-host interactions.

Molecular & general genetics : MGG - 1 Sept 1997

Rainey P B, Heithoff D M, Mahan M J

Abstract excerpt

In vivo expression technology (IVET) is a genetic strategy for isolating genes expressed in vivo. In order to full exploit this technology, it is necessary to analyse large numbers of IVET-generated gene fusions, which must be recovered from the chromosome of host bacteria. In bacteria for which...

Topics

Artificial Gene Fusion
Cloning, Molecular
Conjugation, Genetic
Escherichia coli
Gene Expression
Genes, Bacterial
Genotype
Models, Genetic
Plasmids
Pseudomonas fluorescens
Salmonella typhimurium

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