Article
Single-step conjugative cloning of bacterial gene fusions involved in microbe-host interactions.
Molecular & general genetics : MGG - 1 Sept 1997
Rainey P B, Heithoff D M, Mahan M J
Abstract excerpt
In vivo expression technology (IVET) is a genetic strategy for isolating genes expressed in vivo. In order to full exploit this technology, it is necessary to analyse large numbers of IVET-generated gene fusions, which must be recovered from the chromosome of host bacteria. In bacteria for which transductional methods are not available, the recovery of integrated fusion plasmids is problematic and currently...
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