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Article

Bacteriophage P22 transduction of integrated plasmids: single-step cloning of Salmonella typhimurium gene fusions.

Journal of bacteriology - 1 Nov 1993

Mahan M J, Slauch J M, Mekalanos J J

Abstract excerpt

Transcriptional fusions to Salmonella typhimurium chromosomal genes were constructed by integration of a suicide fusion vector into the chromosome by homologous recombination with random cloned chromosomal fragments. We describe here a transductional method using the generalized transducing phage of S. typhimurium, P22, to clone these fusions directly from the bacterial chromosome, in a single step, without the...

Topics

Bacteriophage P22
Cloning, Molecular
Escherichia coli
Genes, Bacterial
Genotype
Mutagenesis, Insertional
Plasmids
Rec A Recombinases
Recombination, Genetic
Salmonella typhimurium
Transcription, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Transduction, Genetic

Virus Integration

Source

Open in PubMed Open full text

Identifiers

PMID: 8226650
DOI: 10.1128/jb.175.21.7086-7091.1993
PMCID: PMC206837

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8226650-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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