Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Bacteriophage P22 transduction of integrated plasmids: single-step cloning of Salmonella typhimurium gene fusions.

Journal of bacteriology - 1 Nov 1993

Mahan M J, Slauch J M, Mekalanos J J

Abstract excerpt

Transcriptional fusions to Salmonella typhimurium chromosomal genes were constructed by integration of a suicide fusion vector into the chromosome by homologous recombination with random cloned chromosomal fragments. We describe here a transductional method using the generalized transducing phage of S. typhimurium, P22, to clone these fusions directly from the bacterial chromosome, in a single step, without the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cloning, Molecular
Share to Escherichia coli
Share to Genes, Bacterial

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genotype

Share to Mutagenesis, Insertional

Share to Plasmids

Share to Recombination, Genetic

Share to Transcription, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 8226650
DOI: 10.1128/jb.175.21.7086-7091.1993
PMCID: PMC206837

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Bacteriophage P22 transduction of integrated plasmids: single-step cloning of Salmonella typhimurium gene fusions.PMID 8226650Read ↗

Transduction of low-copy number plasmids by bacteriophage P22.Semantic proximity Read ↗

Single-step conjugative cloning of bacterial gene fusions involved in microbe-host interactions.Semantic proximity Read ↗

A Salmonella phage-P22 mutant defective in abortive transduction.Semantic proximity Read ↗

Working with Phage P22.Semantic proximity Read ↗

Inactivation of mismatch repair overcomes the barrier to transduction between Salmonella typhimurium and Salmonella typhi.Semantic proximity Read ↗

Optimizing DNA transduction by selection of mutations that evade bacterial defense systems.Semantic proximity Read ↗

Coupling antitoxins and blue/white screening with parAB/resolvase mutation as a strategy for Salmonella spp. plasmid curing.Semantic proximity Read ↗

Assembling new Escherichia coli strains by transduction using phage P1.Semantic proximity Read ↗

Efficient introduction of cloned mutant alleles into the Escherichia coli chromosome.Semantic proximity Read ↗

Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria.Semantic proximity Read ↗

Evolution of a New Function by Fusion between Phage DNA and a Bacterial Gene.Semantic proximity Read ↗

Approaches to half-tetrad analysis in bacteria: recombination between repeated, inverse-order chromosomal sequences.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.