Article
[Determination of ABO blood group genotypes with one tube PCR reaction].
Zhongguo shi yan xue ye xue za zhi - 1 Dec 2005
Qin Fei, He Ji, Zhu Fa-Ming, Liu Jin-Hui, Chen Shu, Fu Qi-Hua, Yan Li-Xing
Abstract excerpt
This study was aimed to establish one tube PCR reaction technique to determine ABO blood group genotypes. Salting-out method was adopted to extract genomic DNA; one tube polymerase chain reaction with GeneScan technique was used to identify ABO genotypes. The results showed that the ABO genotypes of 132 samples were in accordance with the phenotypes determined by serological technique. The frequencies of A, B and...
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