Article
Multicolor real-time PCR genotyping of ABO system using displacing probes.
Journal of forensic sciences - 1 Jan 2010
Ruan Li, Zhao Huian, Li Qingge
Abstract excerpt
Rapid and informative ABO genotyping has become increasingly popular in forensic use. We developed a multiplex real-time polymerase chain reaction (PCR) approach to genotype ABO major groups and subgroups. Seven differently fluorophor-labeled displacing probes for O(1)(261delG), A(261G), A(796C/803C), B(796A/803C), O(2) (802G>A), A(2) (1059delC), and A(2) (1009A>G) were combined in one or two PCRs to determine...
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