Article
Genotyping of ABO blood groups by PCR and RFLP analysis of 5 nucleotide positions.
International journal of legal medicine - 1 Jan 1995
Fukumori Y, Ohnoki S, Shibata H, Yamaguchi H, Nishimukai H
Abstract excerpt
The genotyping of ABO blood groups was performed using the polymerase chain reaction (PCR) method. The 4 DNA fragments containing the nucleotide position 261, 526, 703 and 796 of cDNA from A-transferase were amplified by PCR, and the amplified DNA subjected to restriction fragment length polymorphism (RFLP) analysis. The different nucleotide at position 803 was clearly distinguished by electrophoresis of the PCR...
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