Article
Statistical Coupling Analysis-Guided Library Design for the Discovery of Mutant Luciferases.
Biochemistry - 6 Feb 2018
Liu Mira D, Warner Elliot A, Morrissey Charlotte E, Fick Caitlyn W, Wu Taia S, Ornelas Marya Y, Ochoa Gabriela V, Zhang Brendan S, Rathbun Colin M, Porterfield William B, Prescher Jennifer A, Leconte Aaron M
Abstract excerpt
Directed evolution has proven to be an invaluable tool for protein engineering; however, there is still a need for developing new approaches to continue to improve the efficiency and efficacy of these methods. Here, we demonstrate a new method for library design that applies a previously developed bioinformatic method, Statistical Coupling Analysis (SCA). SCA uses homologous enzymes to identify amino acid...
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