Role of histidine 46 in the hydrolysis and the reverse transphosphorylation reaction of RNase Rh from Rhizopus niveus.

doi:10.1093/oxfordjournals.jbchem.a021664

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Article

Role of histidine 46 in the hydrolysis and the reverse transphosphorylation reaction of RNase Rh from Rhizopus niveus.

Journal of biochemistry - 1 May 1997

Irie M, Ohgi K, Iwama M, Koizumi M, Sasayama E, Harada K, Yano Y, Udagawa J, Kawasaki M

Abstract excerpt

In order to study the reaction mechanism of RNase Rh from Rhizopus niveus, the rates of cleavage of four 2',3'-cyclic nucleotides by mutant enzymes of RNase Rh, H46F, H109F, E105Q, and K108L were measured. H46F is virtually inactive towards cyclic nucleotides, but H109F hydrolyzed these substrate...

Topics

Binding Sites
Dinucleoside Phosphates
Endoribonucleases
Histidine
Hydrogen-Ion Concentration
Hydrolysis
Kinetics
Mutation
Phosphorylation
Rhizopus

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