Article
The base specificities of tomato ribonuclease (RNase LE) and its Asp44 mutant enzyme expressed from yeast cells.
Bioscience, biotechnology, and biochemistry - 1 Mar 1997
Ohgi K, Shiratori Y, Nakajima A, Iwama M, Kobayashi H, Inokuchi N, Koyama T, Köck M, Löffler A, Glund K, Irie M
Abstract excerpt
RNase LE from cultured tomato cells is a member of the RNase T2 family. It is, however, distinguishable from RNase Rh from Rhizopus niveus, a typical RNase of the RNase T2 family, by its CD spectrum in the 200-250 nm region. In order to reinvestigate the base specificity of RNase LE and to study...
Topics
- Amino Acid Sequence
- Asparagine
- Base Sequence
- Cells, Cultured
- Circular Dichroism
- Electrophoresis, Polyacrylamide Gel
- Endoribonucleases
- Gene Expression Regulation, Enzymologic
- Solanum lycopersicum
- Molecular Sequence Data