Article
pH profile of kinetic constants of RNase Rh from Rhizopus niveus and its mutant enzymes towards UpU, and possible mechanisms of RNase Rh.
Journal of biochemistry - 1 Jun 1994
Irie M, Ohgi K, Watanabe H, Iwama M, Nakamura K T, Kurihara H, Nonaka T, Mitsui Y, Horiuchi H, Takagi M
Abstract excerpt
In order to elucidate the mechanism of action of Rhizopus niveus RNase Rh, we investigated the pH profiles of the kinetic parameters of RNase RNAP Rh, a derivative of RNase Rh, and its mutant enzymes, i.e., RNase RNAP Rh H104F, RNase RNAP Rh E105Q, and RNase RNAP Rh D51N. Based on comparisons of their profiles we concluded that protonation of His104 is indispensable for the enzymatic activity and Glu105...
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