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Article

Reshaping the Activity-Stability Landscape of a 1,2-Rhamnosyltransferase via Distal Mutational Engineering.

Journal of agricultural and food chemistry - 2 Sept 2026

Dai Wenjuan, Guo Chaorong, Yang Hongyan, Chen Taiyan, Qu Jing, Pei Wenwen, Zeng Yan, Zhu Yueming, Qin Hui-Min, Li Jiao, Chen Peng, Sun Yuanxia

Abstract excerpt

Rhamnosyltransferases are remarkable biocatalysts for the synthesis of rhamnosylated natural products with valuable physicochemical properties and bioactivities. However, their application is hindered by poor stability and low catalytic efficiency. Here, we achieved simultaneous enhancement of catalytic efficiency and stability of a 1,2-rhamnosyltransferase by a distal mutational engineering strategy. The variant...

Read the complete abstract on PubMed

Topics

Enzyme Stability
Hexosyltransferases
Protein Engineering
Escherichia coli
Biocatalysis
Kinetics

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Bacterial Proteins

Mutation

Source

Identifiers

PMID: 42690770
DOI: 10.1021/acs.jafc.6c07461

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 23 Oct 2026.

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