Article
p53 protein detected by immunohistochemical staining is not always mutant.
Disease markers - 1 Dec 1993
MacGeoch C, Barnes D M, Newton J A, Mohammed S, Hodgson S V, Ng M, Bishop D T, Spurr N K
Abstract excerpt
The expression of the tumour suppressor gene p53 was analyzed in a variety of human solid tumours by immunohistochemistry and direct DNA sequencing. Positive nuclear staining using a panel of anti-p53 antibodies was used to select tumours for further genetic analysis. Using PCR amplification followed by immobilization onto magnetic beads and direct sequencing, we sequenced exons 5-9 of the p53 gene from 9...
Topics
- Breast Neoplasms
- DNA
- DNA Mutational Analysis
- Female
- Genes, p53
- Humans
- Immunohistochemistry
- Melanoma
- Mutation
- Nasopharyngeal Neoplasms
- Polymerase Chain Reaction