Article
Human insulin gene expression in transgenic mice: mutational analysis of the regulatory region.
Differentiation; research in biological diversity - 1 Sept 1996
Itier J M, Douhet P, Desbois P, Joshi R L, Dandoy-Dron F, Jami J, Bucchini D
Abstract excerpt
A mini-human insulin gene and four derivatives mutated at several regions potentially involved in the regulation of gene expression were used to generate transgenic mouse lines. The effect of these mutations on the efficiency of gene expression and cell specificity was studied using three approaches: (1) Northern blot analysis using total RNA from pancreas and other organs, (2) radioimmunoassay to detect the...
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