Article
GGAAAT motifs play a major role in transcriptional activity of the human insulin gene in a pancreatic islet beta-cell line MIN6.
Diabetologia - 1 Dec 1996
Tomonari A, Yoshimoto K, Tanaka M, Iwahana H, Miyazaki J, Itakura M
Abstract excerpt
The insulin gene is specifically expressed in pancreatic islet beta cells. Various cis-acting DNA elements in the 5'-flanking region of the human insulin gene were examined for their contribution to the transcriptional activity using sensitive human growth hormone (hGH) reporter plasmids. The hGH...
Topics
- 3T3 Cells
- Animals
- Base Sequence
- Cell Line
- DNA Probes
- Gene Deletion
- Human Growth Hormone
- Humans
- Insulin
- Islets of Langerhans
- Mice
- Mutation
- Polymerase Chain Reaction
- Promoter Regions, Genetic
- Transcriptional Activation
- Transfection