Article
Positive and negative regulation of the human insulin gene by multiple trans-acting factors.
The Journal of biological chemistry - 15 May 1990
Boam D S, Clark A R, Docherty K
Abstract excerpt
Tissue-specific expression of the human insulin gene is regulated by cis-acting DNA elements 5' to the transcription start site. Deletion of the 5' region of the human insulin gene between nucleotides -279 and -258 caused a 25-fold rise in transcriptional activity whereas further deletion to nucleotide -229 reduced transcription activity 25-fold. In vitro analysis of protein binding in the 5' regulatory region...
Topics
- Base Sequence
- Binding Sites
- Chloramphenicol O-Acetyltransferase
- Down-Regulation
- Gene Expression Regulation
- Humans
- Insulin
- Islets of Langerhans
- Molecular Sequence Data
- Mutation
- Regulatory Sequences, Nucleic Acid