Article
Contribution of a tyrosine side chain to ribonuclease A catalysis and stability.
Protein science : a publication of the Protein Society - 1 Aug 1996
Eberhardt E S, Wittmayer P K, Templer B M, Raines R T
Abstract excerpt
An intricate architecture of covalent bonds and noncovalent interactions appear to position the side chain of Lys 41 properly within the active site of bovine pancreatic ribonuclease A (RNase A). One of these interactions arises from Tyr 97, which is conserved in all 41 RNase A homologues of know...
Topics
- Animals
- Catalysis
- Cattle
- Enzyme Stability
- Escherichia coli
- Hot Temperature
- Hydrogen Bonding
- Kinetics
- Mutation
- Pancreas
- Phosphorylation
- Protein Binding
- Protein Denaturation
- Recombinant Proteins
- Ribonuclease, Pancreatic
- Structure-Activity Relationship
- Thermodynamics
- Tyrosine