Stabilization of the transition state for the transfer of tyrosine to tRNA(Tyr) by tyrosyl-tRNA synthetase.

doi:10.1006/jmbi.2000.4126

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Article

Stabilization of the transition state for the transfer of tyrosine to tRNA(Tyr) by tyrosyl-tRNA synthetase.

Journal of molecular biology - 20 Oct 2000

Xin Y, Li W, First E A

Abstract excerpt

Aminoacylation of tRNA(Tyr) involves two steps: (1) tyrosine activation to form the tyrosyl-adenylate intermediate; and (2) transfer of tyrosine from the tyrosyl-adenylate intermediate to tRNA(Tyr). In Bacillus stearothermophilus tyrosyl-tRNA synthetase, Asp78, Tyr169, and Gln173 have been shown to form hydrogen bonds with the alpha-ammonium group of the tyrosine substrate during the first step of the...

Topics

Acylation
Adenosine
Adenosine Triphosphate
Amino Acid Substitution
Aspartic Acid
Catalysis
Enzyme Stability
Evolution, Molecular
Geobacillus stearothermophilus
Glutamine
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Protein Binding

RNA, Bacterial

RNA, Transfer, Amino Acyl

Thermodynamics

Tyrosine

Tyrosine-tRNA Ligase

Source

Open in PubMed

Identifiers

PMID: 11023794
DOI: 10.1006/jmbi.2000.4126

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