Article
The crystal structure of a mutant human lysozyme C77/95A with increased secretion efficiency in yeast.
The Journal of biological chemistry - 5 Jul 1991
Inaka K, Taniyama Y, Kikuchi M, Morikawa K, Matsushima M
Abstract excerpt
The three-dimensional structure of a mutant human lysozyme, C77/95A, in which residues Cys77 and Cys95 were replaced by alanine, was determined at 1.8-A resolution by x-ray crystallography. The properties of this mutant protein have been well characterized with respect to its thermal stability an...
Topics
- Alanine
- Crystallization
- Cysteine
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Hot Temperature
- Humans
- Models, Molecular
- Muramidase
- Mutation
- Protein Conformation
- Saccharomyces cerevisiae
- X-Ray Diffraction
