Contribution of histidine residues to the conformational stability of ribonuclease T1 and mutant Glu-58----Ala.

doi:10.1021/bi00485a005

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Article

Contribution of histidine residues to the conformational stability of ribonuclease T1 and mutant Glu-58----Ala.

Biochemistry - 21 Aug 1990

McNutt M, Mullins L S, Raushel F M, Pace C N

Abstract excerpt

The pK values of the histidine residues in ribonuclease T1 (RNase T1) are unusually high: 7.8 (His-92), 7.9 (His-40), and 7.3 (His-27) [Inagaki et al. (1981) J. Biochem. 89, 1185-1195]. In the RNase T1 mutant Glu-58----Ala, the first two pK values are reduced to 7.4 (His-92) and 7.1 (His-40). The...

Topics

Alanine
Enzyme Stability
Glutamates
Glutamic Acid
Histidine
Hydrogen-Ion Concentration
Mutation
Protein Conformation
Ribonuclease T1
Thermodynamics

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