Article
Single nucleotide primer extension: quantitative range, variability, and multiplex analysis.
Genome research - 1 Apr 1996
Greenwood A D, Burke D T
Abstract excerpt
The quantitative measurement of transcription products from homologous alleles at a diploid locus has broad application for the study of mammalian gene expression. Single nucleotide primer extension (SNuPE) analysis is a simple and sensitive method for allelic transcript discrimination requiring...
Topics
- Alleles
- Animals
- Base Sequence
- DNA Primers
- Hypoxanthine Phosphoribosyltransferase
- Menkes Kinky Hair Syndrome
- Mice
- Mice, Inbred C57BL
- Molecular Sequence Data
- Ornithine Carbamoyltransferase
- Poly T
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Ribonucleases
- Sensitivity and Specificity
- Species Specificity
- Transcription, Genetic