Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.

doi:10.1677/jme.0.0250169

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Article

Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.

Journal of molecular endocrinology - 1 Oct 2000

Bustin S A

Abstract excerpt

The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method for the detection of low-abundance mRNA, often obtained from limited tissue samples. However, it is a complex technique, there are substantial problems associated with its true sensitivity, reproducibility and specificity and, as a quantitative method, it suffers from the problems inherent in PCR. The recent introduction of...

Topics

Actins
Alleles
Alternative Splicing
Costs and Cost Analysis
DNA
DNA Primers
Fluorescent Dyes
Glyceraldehyde-3-Phosphate Dehydrogenases
Humans
Hydrolysis
Kinetics
Molecular Probes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Nucleic Acid Hybridization

RNA, Messenger

RNA, Ribosomal

Reproducibility of Results

Reverse Transcriptase Polymerase Chain Reaction

Source

Open in PubMed

Identifiers

PMID: 11013345
DOI: 10.1677/jme.0.0250169

Genes

GAPDH

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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