Article
Analysis of variation in relative mRNA abundance for specific gene transcripts in single bovine oocytes and early embryos.
Molecular reproduction and development - 1 Feb 1998
De Sousa P A, Westhusin M E, Watson A J
Abstract excerpt
Variation in the abundance of a specific gene transcript was assessed in single bovine oocytes and in vitro-derived blastocysts. Transcripts encoding the Na+,K(+)-ATPase alpha 1 subunit were detected by reverse-transcription polymerase chain reaction (RT-PCR) and quantified relative to an exogenously supplied rabbit alpha-globin mRNA using laser-induced fluorescence capillary electrophoresis (LIF-CE). The...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
