Article
Mutational analysis of the Escherichia coli spoT gene identifies distinct but overlapping regions involved in ppGpp synthesis and degradation.
Molecular microbiology - 1 Mar 1996
Gentry D R, Cashel M
Abstract excerpt
The spoT gene of Escherichia coli encodes a guanosine 3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (ppGppase) as well as an apparent guanosine 3',5'-bis(diphosphate) synthetase (designated PSII). To determine the regions of the SpoT protein that are required for these two competing activities, we analysed plasmid-borne deletion mutations for their ability to complement chromosomal mutations defective in each...
Topics
- Base Sequence
- DNA Mutational Analysis
- DNA Primers
- DNA, Bacterial
- Escherichia coli
- Gene Deletion
- Genes, Bacterial
- Guanosine Tetraphosphate
- Ligases
- Molecular Sequence Data
- Molecular Structure
- Mutation
- Plasmids
- Pyrophosphatases