Article
Temperature-sensitive growth and decreased thermotolerance associated with relA mutations in Escherichia coli.
Journal of bacteriology - 1 Oct 2003
Yang Xiaoming, Ishiguro Edward E
Abstract excerpt
The relA gene of Escherichia coli encodes guanosine 3',5'-bispyrophosphate (ppGpp) synthetase I, a ribosome-associated enzyme that is activated during amino acid starvation. The stringent response is thought to be mediated by ppGpp. Mutations in relA are known to result in pleiotropic phenotypes. We now report that three different relA mutant alleles, relA1, relA2, and relA251::kan, conferred...
Topics
- Culture Media
- DNA-Directed RNA Polymerases
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Ligases
- Mutation
- Pyrophosphatases
- Sodium Chloride
- Temperature