Article
Systematic production of inactivating and non-inactivating suppressor mutations at the relA locus that compensate the detrimental effects of complete spot loss and affect glycogen content in Escherichia coli.
PloS one - 1 Jan 2014
Montero Manuel, Rahimpour Mehdi, Viale Alejandro M, Almagro Goizeder, Eydallin Gustavo, Sevilla Ángel, Cánovas Manuel, Bernal Cristina, Lozano Ana Belén, Muñoz Francisco José, Baroja-Fernández Edurne, Bahaji Abdellatif, Mori Hirotada, Codoñer Francisco M, Pozueta-Romero Javier
Abstract excerpt
In Escherichia coli, ppGpp is a major determinant of growth and glycogen accumulation. Levels of this signaling nucleotide are controlled by the balanced activities of the ppGpp RelA synthetase and the dual-function hydrolase/synthetase SpoT. Here we report the construction of spoT null (ΔspoT) mutants obtained by transducing a ΔspoT allele from ΔrelAΔspoT double mutants into relA+ cells. Iodine staining of...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.