Article
Guanosine tetraphosphate (ppGpp) dependence of the growth rate control of rrnB P1 promoter activity in Escherichia coli.
The Journal of biological chemistry - 15 Jul 1990
Hernandez V J, Bremer H
Abstract excerpt
We have fused the ribosomal RNA promoter P1 from the rrnB gene of Escherichia coli to lacZ and examined its guanosine tetraphosphate (ppGpp)-dependent expression at different growth rates. The rrnB P1-lacZ fusion was constructed on plasmid vectors and then recombined into the chromosome of a delt...
Topics
- Chloramphenicol
- Culture Media
- Escherichia coli
- Genes, Bacterial
- Genotype
- Guanine Nucleotides
- Guanosine Tetraphosphate
- Kinetics
- Models, Theoretical
- Plasmids
- Promoter Regions, Genetic
- RNA, Ribosomal
- Restriction Mapping
- beta-Galactosidase