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Article

Identification of an indispensable amino acid for ppGpp synthesis of Escherichia coli SpoT protein.

Bioscience, biotechnology, and biochemistry - 1 Dec 2002

Fujita Chizuko, Maeda Maki, Fujii Takako, Iwamoto Ryoko, Ikehara Kenji

Abstract excerpt

Amino acid substitutions were introduced into a structurally flexible and highly conserved region of Escherichia coli SpoT protein. SpoT protein changed from Asp to Ala at the 293rd position did not restore cell growth of E. coli CF8295 (delta relA, delta spoT) and did not accumulate ppGpp in the cell, suggesting that the Asp293 is indispensable for ppGpp synthesis of the protein.

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Source

Identifiers

PMID: 12596879
DOI: 10.1271/bbb.66.2735

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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