Article
Escherichia coli ppGpp synthetase II activity requires spoT.
The Journal of biological chemistry - 25 Mar 1991
Hernandez V J, Bremer H
Abstract excerpt
Escherichia coli has two enzymes catalyzing the synthesis of guanosine tetraphosphate (ppGpp), designated ppGpp synthetase I (PSI = RelA) and II (PSII), whose activities are regulated differently. Until now, the gene for PSII had not been identified. Here, an E. coli relA1 strain that expresses lacZ from an rrnB P1 promoter was used to screen mutants with increased beta-galactosidase activity on...
Topics
- Bacteriophages
- DNA, Bacterial
- Escherichia coli
- Genes, Viral
- Genetic Complementation Test
- Guanosine Tetraphosphate
- Mutation
- Polymerase Chain Reaction
- Promoter Regions, Genetic
- Species Specificity