Article
Simultaneous assessment of loss of heterozygosity at multiple microsatellite loci using semi-automated fluorescence-based detection: subregional mapping of chromosome 4 in cervical carcinoma.
Proceedings of the National Academy of Sciences of the United States of America - 25 Jun 1996
Hampton G M, Larson A A, Baergen R N, Sommers R L, Kern S, Cavenee W K
Abstract excerpt
Detection of loss of heterozygosity (LOH) by comparison of normal and tumor genotypes using PCR-based microsatellite loci provides considerable advantages over traditional Southern blotting-based approaches. However, current methodologies are limited by several factors, including the numbers of loci that can be evaluated for LOH in a single experiment, the discrimination of true alleles versus "stutter bands,"...
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